Transformation Lab Fall 2011
Students performed the TRANSFORMATION LAB and did a remarkable job making an organism that does not exist in nature.
They transformed competent E. coli cells by enticing them to uptake an genetically engineered plasmid that contained a gene from a jellyfish (pGLO) that glows under UV light.
In addition to the pGLO gene, the plasmid contained an AMP' gene that makes transformed cells resistent to ampicillin. Thus, with ampicillin in the agar, the only cells that remained were those that were transformed.
Below are pictures of the results as well as some of the students performing the experiment: